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MRP-L21 Polyclonal Antibody
Datasheet
Catalog Number:
E20-72853Amount:
100ulMolecular Weight:
23159DaApplications:
WB;IHC-p;ELISA;IF(paraffin section)Reactivity:
Human;MousePurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human MRPL21. AA range:131-180Source:
Polyclonal, Rabbit,IgG
- Western Blot analysis of various cells using MRP-L21 Polyclonal Antibody diluted at 1:2000
Gene Name:
MRPL21Dilution:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.Protein Name:
39S ribosomal protein L21 mitochondrialHuman Gene Id:
219927Human Swiss Prot No:
Q7Z2W9Mouse Gene Id:
353242Mouse Swiss Prot No:
Q9D1N9Specificity:
MRP-L21 Polyclonal Antibody detects endogenous levels of MRP-L21 protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
MRPL21; 39S ribosomal protein L21; mitochondrial; L21mt; MRP-L21Observed Band(KD):
23Background:
mitochondrial ribosomal protein L21(MRPL21) Homo sapiens Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Multiple transcript variants encoding different isoforms were identified through sequence analysis although some may be subject to nonsense-mediated decay (NMD). [provided by RefSeq, Jul 2008],Function:
similarity:Belongs to the ribosomal protein L21P family.,Subcellular Location:
Mitochondrion .
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MRPL21 Antibody. The picture on the right is blocked with the synthesized peptide.
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