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MRP-L20 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72852

  • Amount:

    100ul

  • Molecular Weight:

    17443Da

  • Applications:

    WB;IHC-p;ELISA;IF(paraffin section)

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human MRPL20. AA range:100-149

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using MRP-L20 Polyclonal Antibody

  • Gene Name:

    MRPL20

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

  • Protein Name:

    39S ribosomal protein L20 mitochondrial

  • Human Gene Id:

    55052

  • Human Swiss Prot No:

    Q9BYC9

  • Mouse Gene Id:

    66448

  • Mouse Swiss Prot No:

    Q9CQL4

  • Specificity:

    MRP-L20 Polyclonal Antibody detects endogenous levels of MRP-L20 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    MRPL20; 39S ribosomal protein L20; mitochondrial; L20mt; MRP-L20

  • Observed Band(KD):

    22

  • Background:

    mitochondrial ribosomal protein L20(MRPL20) Homo sapiens Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 21q. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016],

  • Function:

    similarity:Belongs to the ribosomal protein L20P family.,

  • Subcellular Location:

    Mitochondrion .

  • Expression:

    Bone marrow,Lymph,Mammary gland,Whole embryo,

  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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