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MRLC2 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72839
  • Amount:

    100ul
  • Molecular Weight:

    19663Da
  • Applications:

    WB;IHC-p;IF/ICC;ELISA
  • Reactivity:

    Human;Mouse;Rat
  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human MRLC2. AA range:3-52
  • Source:

    Polyclonal, Rabbit,IgG
  • Western Blot analysis of various cells using MRLC2 Polyclonal Antibody
  • Gene Name:

    MLRN
  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
  • Protein Name:

    Myosin regulatory light polypeptide 9
  • Human Gene Id:

    10398/10627
  • Human Swiss Prot No:

    P24844/P19105
  • Mouse Gene Id:

    98932
  • Rat Gene Id:

    296313
  • Rat Swiss Prot No:

    Q64122
  • Specificity:

    MRLC2 Polyclonal Antibody detects endogenous levels of MRLC2 protein.
  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Concentration:

    1 mg/ml
  • Storage Stability:

    -20°C/1 year
  • Other Name:

    MYL9; MLC2; MRLC1; MYRL2; Myosin regulatory light polypeptide 9; 20 kDa myosin light chain; LC20; MLC-2C; Myosin RLC; Myosin regulatory light chain 2; smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRL
  • Observed Band(KD):

    18
  • Background:

    myosin light chain 9(MYL9) Homo sapiens Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
  • Function:

    function:Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.,miscellaneous:This chain binds calcium.,PTM:Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.,similarity:Contains 3 EF-hand domains.,subunit:Myosin is an hexamer of 2 heavy chains and 4 light chains.,tissue specificity:Smooth muscle tissues and in some, but not all, nonmuscle cells.,
  • Subcellular Location:

    Cytoplasm, cytoskeleton . Cytoplasm, cell cortex . Colocalizes with F-actin, MYH9 and PIEZO1 at the actomyosin cortex in myoblasts. .
  • Expression:

    Smooth muscle tissues and in some, but not all, nonmuscle cells.
  • Immunofluorescence analysis of HUVEC cells, using MRLC2 Antibody. The picture on the right is blocked with the synthesized peptide.

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Catalog Number

  • Amount:100ul
  • Price:225$

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AbPromise
MRLC2 Polyclonal Antibody | EnoGene Biotech Co. Ltd