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MRCKβ Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72828

  • Amount:

    100ul

  • Molecular Weight:

    194315Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human MRCKB. AA range:1641-1690

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using MRCKβ Polyclonal Antibody

  • Gene Name:

    CDC42BPB

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

  • Protein Name:

    Serine/threonine-protein kinase MRCK beta

  • Human Gene Id:

    9578

  • Human Swiss Prot No:

    Q9Y5S2

  • Mouse Gene Id:

    217866

  • Mouse Swiss Prot No:

    Q7TT50

  • Rat Gene Id:

    113960

  • Rat Swiss Prot No:

    Q7TT49

  • Specificity:

    MRCKβ Polyclonal Antibody detects endogenous levels of MRCKβ protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CDC42BPB; KIAA1124; Serine/threonine-protein kinase MRCK beta; CDC42-binding protein kinase beta; CDC42BP-beta; DMPK-like beta; Myotonic dystrophy kinase-related CDC42-binding kinase beta; MRCK beta; Myotonic dystrophy protein kinase-like b

  • Observed Band(KD):

    194

  • Background:

    This gene encodes a member of the serine/threonine protein kinase family. The encoded protein contains a Cdc42/Rac-binding p21 binding domain resembling that of PAK kinase. The kinase domain of this protein is most closely related to that of myotonic dystrophy kinase-related ROK. Studies of the similar gene in rat suggested that this kinase may act as a downstream effector of Cdc42 in cytoskeletal reorganization. [provided by RefSeq, Jul 2008],

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation.,function:May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.,similarity:Conta

  • Subcellular Location:

    Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction . Cell projection, lamellipodium . Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent (By similarity). Detected at the leading edge of migrating cells. Localization at the leading edge of migrating cells requires interaction with catalytically active CDC42 (PubMed:21240187). Localizes in the lamellipodium in a FAM89B/LRAP25-dependent manner (By similarity). .

  • Expression:

    Expressed in all tissues examined, with high levels in heart, brain, placenta and lung.

  • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MRCKB Antibody. The picture on the right is blocked with the synthesized peptide.

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