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MRCKα Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72827

  • Amount:

    100ul

  • Molecular Weight:

    197307Da

  • Applications:

    WB;ELISA;IHC-p

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    Synthesized peptide derived from the Internal region of human MRCKα.

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using MRCKα Polyclonal Antibody

  • Gene Name:

    CDC42BPA

  • Dilution:

    WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000

  • Protein Name:

    Serine/threonine-protein kinase MRCK alpha

  • Human Gene Id:

    8476

  • Human Swiss Prot No:

    Q5VT25

  • Mouse Swiss Prot No:

    Q3UU96

  • Rat Gene Id:

    114116

  • Rat Swiss Prot No:

    O54874

  • Specificity:

    MRCKα Polyclonal Antibody detects endogenous levels of MRCKα protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CDC42BPA; KIAA0451; Serine/threonine-protein kinase MRCK alpha; CDC42-binding protein kinase alpha; DMPK-like alpha; Myotonic dystrophy kinase-related CDC42-binding kinase alpha; MRCK alpha; Myotonic dystrophy protein kinase-like alpha

  • Observed Band(KD):

    200

  • Background:

    The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported. [provided by RefSeq, Jul 2008],

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation.,function:May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 CNH domain

  • Subcellular Location:

    Cytoplasm . Cell projection, lamellipodium . Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent. Localizes in the lamellipodium in a FAM89B/LRAP25-dependent manner. .

  • Expression:

    Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver.

  • Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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Catalog Number

  • Amount:100ul
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MRCKα Polyclonal Antibody | EnoGene Biotech Co. Ltd