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MNT Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72809

  • Amount:

    100ul

  • Molecular Weight:

    62300Da

  • Applications:

    WB;ELISA;IHC-p

  • Reactivity:

    Human;Rat;Mouse;

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human MNT. AA range:315-364

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using MNT Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

  • Gene Name:

    MNT

  • Dilution:

    WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000

  • Protein Name:

    Max-binding protein MNT

  • Human Gene Id:

    4335

  • Human Swiss Prot No:

    Q99583

  • Mouse Swiss Prot No:

    O08789

  • Specificity:

    MNT Polyclonal Antibody detects endogenous levels of MNT protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    MNT; BHLHD3; ROX; Max-binding protein MNT; Class D basic helix-loop-helix protein 3; bHLHd3; Myc antagonist MNT; Protein ROX

  • Observed Band(KD):

    62

  • Background:

    The Myc/Max/Mad network comprises a group of transcription factors that co-interact to regulate gene-specific transcriptional activation or repression. This gene encodes a protein member of the Myc/Max/Mad network. This protein has a basic-Helix-Loop-Helix-zipper domain (bHLHzip) with which it binds the canonical DNA sequence CANNTG, known as the E box, following heterodimerization with Max proteins. This protein is likely a transcriptional repressor and an antagonist of Myc-dependent transcriptional activation and cell growth. This protein represses transcription by binding to DNA binding proteins at its N-terminal Sin3-interaction domain. [provided by RefSeq, Jul 2008],

  • Function:

    function:Binds DNA as a heterodimer with MAX and represses transcription. Binds to the canonical E box sequence 5'-CACGTG-3' and, with higher affinity, to 5'-CACGCG-3'.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as an homodimer or a heterodimer with MAX.,

  • Subcellular Location:

    Nucleus.

  • Expression:

    Fetal brain,Pancreas,

  • Western Blot analysis of A549 cells using MNT Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

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