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MIF Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72761

  • Amount:

    100ul

  • Molecular Weight:

    12476Da

  • Applications:

    IF/ICC;WB;IHC-p;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human MIF. AA range:25-74

  • Source:

    Polyclonal, Rabbit,IgG

  • Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.

  • Gene Name:

    MIF

  • Dilution:

    IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300

  • Protein Name:

    Macrophage migration inhibitory factor

  • Human Gene Id:

    4282

  • Human Swiss Prot No:

    P14174

  • Mouse Gene Id:

    17319

  • Mouse Swiss Prot No:

    P34884

  • Rat Gene Id:

    81683

  • Rat Swiss Prot No:

    P30904

  • Specificity:

    MIF Polyclonal Antibody detects endogenous levels of MIF protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    MIF; GLIF; MMIF; Macrophage migration inhibitory factor; MIF; Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase

  • Observed Band(KD):

    12

  • Background:

    This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008],

  • Function:

    catalytic activity:Keto-phenylpyruvate = enol-phenylpyruvate.,disease:Genetic variations in MIF are associated with susceptibility to systemic juvenile rheumatoid arthritis [MIM:604302]. Systemic juvenile rheumatoid arthritis is juvenile chronic arthritis associated with severe, debilitating, extraarticular features, and occasionally fatal complications. Despite medical treatment, many children still experience early joint destruction, necessitating surgical replacement.,function:The expression of MIF at sites of inflammation suggest a role for the mediator in regulating the function of macrophage in host defense. Also acts as a phenylpyruvate tautomerase.,similarity:Belongs to the MIF family.,subunit:Homotrimer. Interacts with COPS5 and BNIPL.,

  • Subcellular Location:

    Secreted . Cytoplasm . Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. .

  • Expression:

    Brain,Lens,Liver,Lung,Platelet,SkinLC3-II may mediate ATR-induced mitophagy in dopaminergic neurons through SQSTM1/p62 pathway. ACTA BIOCHIMICA ET BIOPHYSICA SINICA 2018 Oct 01 WB Rat 1:1000 substantia nigra (SNpc) PC-12 cell MIF, secreted by human hepatic sinusoidal endothelial cells, promotes chemotaxis and outgrowth of colorectal cancer in liver prometastasis. Oncotarget Oncotarget. 2015 Sep 8; 6(26): 22410–22423 IHC Human 1:100 CRC tissue

  • Immunofluorescence analysis of Hela cell. 1,MIF Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.

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Catalog Number

  • Amount:100ul
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