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MGMT Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72750

  • Amount:

    100ul

  • Molecular Weight:

    21646Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human MGMT. AA range:1-50

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using MGMT Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

  • Gene Name:

    MGMT

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

  • Protein Name:

    Methylated-DNA--protein-cysteine methyltransferase

  • Human Gene Id:

    4255

  • Human Swiss Prot No:

    P16455

  • Mouse Swiss Prot No:

    P26187

  • Rat Gene Id:

    25332

  • Rat Swiss Prot No:

    P24528

  • Specificity:

    MGMT Polyclonal Antibody detects endogenous levels of MGMT protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    MGMT; Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

  • Observed Band(KD):

    22

  • Background:

    Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015],

  • Function:

    catalytic activity:DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.,cofactor:Binds 1 zinc ion.,function:Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.,similarity:Belongs to the MGMT family.,

  • Subcellular Location:

    Nucleus.

  • Expression:

    Cerebellum,Epithelium,Placenta,

  • Western Blot analysis of Jurkat cells using MGMT Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

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