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Met Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72738

  • Amount:

    100ul

  • Molecular Weight:

    155527Da

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Met. AA range:1316-1365

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Met Polyclonal Antibody

  • Gene Name:

    MET

  • Dilution:

    IHC-p: 100-300.Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

  • Protein Name:

    Hepatocyte growth factor receptor

  • Human Gene Id:

    4233

  • Human Swiss Prot No:

    P08581

  • Mouse Swiss Prot No:

    P16056

  • Rat Gene Id:

    24553

  • Rat Swiss Prot No:

    P97523

  • Specificity:

    Met Polyclonal Antibody detects endogenous levels of Met protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    MET; Hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met

  • Observed Band(KD):

    145

  • Background:

    This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. [provided by RefSeq, May 2016],

  • Function:

    catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:Activation of MET after rearrangement with the TPR gene produces an oncogenic protein.,disease:Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550].,disease:Defects in MET are a cause of hereditary papillary renal carcinoma (HPRC) [MIM:605074]; also known as papillary renal cell carcinoma 2 (RCCP2). HPRC is a form of inherited kidney cancer characterized by a predisposition to develop multiple, bilateral papillary renal tumors. The pattern of inheritance is consistent with autosomal dominant transmission with reduced penetrance.,disease:Defects in MET may be associated with gastric cancer.,disease:Genetic variations in MET may be associated with susceptibility to autism type 9 (AUTS9) [MIM:611015]. Autism is a neurodevelopmental disorder characterized by disturbance in l

  • Subcellular Location:

    Membrane; Single-pass type I membrane protein.; [Isoform 3]: Secreted.

  • Expression:

    Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Found also in basal keratinocytes of esophagus and skin. High levels are found in liver, gastrointestinal tract, thyroid and kidney. Also present in the brain. Expressed in metaphyseal bone (at protein level) (PubMed:26637977).miR-221 and miR-222 synergistically regulate hepatocyte growth factor activator inhibitor type 1 to promote cell proliferation and migration in gastric cancer:. TUMOR BIOLOGY 2017 Jun 15 WB Human 1:2000 GC cancer tissues MGC-803 cell

  • Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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Catalog Number

  • Amount:100ul
  • Price:225$

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Met Polyclonal Antibody | EnoGene Biotech Co. Ltd