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MEK-1/2 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72715

  • Amount:

    100ul

  • Molecular Weight:

    43439Da

  • Applications:

    IF/ICC;WB;IHC-p;ELISA

  • Reactivity:

    Human;Mouse;Rat;Monkey

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human MEK1/2. AA range:189-238

  • Source:

    Polyclonal, Rabbit,IgG

  • Immunofluorescence analysis of Hela cell. 1,MEK-1/2 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). CYCS Monoclonal Antibody(4B10)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).

  • Gene Name:

    MAP2K1/MAP2K2

  • Dilution:

    IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300

  • Protein Name:

    Dual specificity mitogen-activated protein kinase kinase 1/2

  • Human Gene Id:

    5604/5605

  • Human Swiss Prot No:

    Q02750/P36507

  • Mouse Gene Id:

    26395/26396

  • Rat Gene Id:

    170851/58960

  • Rat Swiss Prot No:

    Q01986/P36506

  • Specificity:

    MEK-1/2 Polyclonal Antibody detects endogenous levels of MEK-1/2 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    MAP2K1; MEK1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; MAP2K2; MEK2; MKK2; PRKMK2; Dual specificity mitogen-activated protein k

  • Observed Band(KD):

    43

  • Background:

    The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008],

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.,enzyme reg

  • Subcellular Location:

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body . Cytoplasm . Nucleus . Membrane ; Peripheral membrane protein . Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis (PubMed:14737111). Membrane localization is probably regulated by its interaction with KSR1 (PubMed:10409742). .

  • Expression:

    Widely expressed, with extremely low levels in brain.Effects of hydroxysafflor yellow A on rats with collagen-induced arthritis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Biochem Bioph Res Co. 2021 Sep;570:26 WB Rat 1:500 Synovial CPNE1 silencing inhibits the proliferation, invasion and migration of human osteosarcoma cells. ONCOLOGY REPORTS 2017 Dec 04 WB Human 1:1000 Saos-2 cell

  • Immunofluorescence analysis of rat-lung tissue. 1,MEK-1/2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

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