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MEK Kinase-3 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72708

  • Amount:

    100ul

  • Molecular Weight:

    70898Da

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human MAP3K3. AA range:101-150

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using MEK Kinase-3 Polyclonal Antibody diluted at 1:2000

  • Gene Name:

    MAP3K3

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

  • Protein Name:

    Mitogen-activated protein kinase kinase kinase 3

  • Human Gene Id:

    4215

  • Human Swiss Prot No:

    Q99759

  • Mouse Gene Id:

    26406

  • Mouse Swiss Prot No:

    Q61084

  • Specificity:

    MEK Kinase-3 Polyclonal Antibody detects endogenous levels of MEK Kinase-3 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    MAP3K3; MAPKKK3; MEKK3; Mitogen-activated protein kinase kinase kinase 3; MAPK/ERK kinase kinase 3; MEK kinase 3; MEKK 3

  • Observed Band(KD):

    71

  • Background:

    This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by phosphorylation on Thr-530.,function:Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 OPR domain.,similarity:Contains 1 protein kinase domain.,subunit:Binds both upstream activators and downstream substrates in multimolecular complexes. Part of a complex with MAP2K3, RAC1 and CCM2. Interacts with MAP2K5 and SPAG9.,

  • Subcellular Location:

    cytoplasm,cytosol,

  • Expression:

    Aorta,Brain,Epithelium,Kidney,Melanoma,Placenta,PNS

  • Western blot analysis of lysates from HepG2 cells, using MAP3K3 Antibody. The lane on the right is blocked with the synthesized peptide.

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