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Med18 Polyclonal Antibody
Datasheet
Catalog Number:
E20-72696Amount:
100ulMolecular Weight:
23663 DaObserved Band:24 KDApplications:
WB;ELISAReactivity:
Human;Mouse;RatPurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human MED18. AA range:70-119Source:
Polyclonal, Rabbit,IgG
- Western Blot analysis of various cells using Med18 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
Gene Name:
MED18Dilution:
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.Protein Name:
Mediator of RNA polymerase II transcription subunit 18Human Gene Id:
54797Human Swiss Prot No:
Q9BUE0Mouse Gene Id:
67219Mouse Swiss Prot No:
Q9CZ82Specificity:
Med18 Polyclonal Antibody detects endogenous levels of Med18 protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
MED18; Mediator of RNA polymerase II transcription subunit 18; Mediator complex subunit 18; p28bBackground:
mediator complex subunit 18(MED18) Homo sapiens MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied by OMIM, Oct 2008],Function:
function:Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.,similarity:Belongs to the Mediator complex subunit 18 family.,subunit:Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 suSubcellular Location:
Nucleus .Expression:
Colon,Lymphoma,Uterus,
- Western blot analysis of lysates from COLO and Jurkat cells, using MED18 Antibody. The lane on the right is blocked with the synthesized peptide.
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