World supplier of antibodies, antigens and kits since 2007
Cell Signal Transduction Research Antibodies
MDM2 Polyclonal Antibody
Human;Mouse;Monkey
WB;IHC-p;IF/ICC;ELISA
Polyclonal, Rabbit,IgG
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight55233 DaObserved Band:90 KD
Protein NameE3 ubiquitin-protein ligase Mdm2
Human Gene Id4193
Human Swiss Prot NoQ00987
Mouse Gene Id17246
Mouse Swiss Prot NoP23804
SpecificityMDM2 Polyclonal Antibody detects endogenous levels of MDM2 protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameMDM2; E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2
Functiondisease:Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Four of the splice variants show loss of p53 binding.,domain:Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73 and E2F1. Region II contains most of a central acidic region required for interaction with ribosomal protein L5 and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc interacts specifically with RNA whether or not zinc is present and mediates the heterooligomerization with MDM4. It is also essential for its ubiquitin ligase E3 activity toward p53 and itself.,function:Inhibits TP53/p53- and TP73/p73-mediated cell cycle arrest
Subcellular LocationNucleus, nucleoplasm. Cytoplasm . Nucleus, nucleolus. Nucleus . Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.
ExpressionUbiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from human MDM2. AA range:132-181
Gene NameMDM2
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
BackgroundMDM2 proto-oncogene(MDM2) Homo sapiens This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013],
Conjugated Antibody
This product is also available conjugated. Format: E20-72692-Conjugate. Price: $485 / Size: 100μl.
E20-72692-FITC$485 / 100μlE20-72692-Biotin$485 / 100μlE20-72692-HRP$485 / 100μlE20-72692-AP$485 / 100μlE20-72692-AF350$485 / 100μlE20-72692-AF405M$485 / 100μlE20-72692-AF488$485 / 100μlE20-72692-AF514$485 / 100μlE20-72692-AF532$485 / 100μlE20-72692-AF555$485 / 100μlE20-72692-AF568$485 / 100μlE20-72692-AF405S$485 / 100μlE20-72692-AF405L$485 / 100μlE20-72692-AF546$485 / 100μlE20-72692-AF594$485 / 100μlE20-72692-AF610$485 / 100μlE20-72692-AF635$485 / 100μlE20-72692-AF647$485 / 100μlE20-72692-AF680$485 / 100μlE20-72692-AF700$485 / 100μlE20-72692-AF750$485 / 100μlE20-72692-AF790$485 / 100μlE20-72692-APC$485 / 100μlE20-72692-PE$485 / 100μlE20-72692-Cy3$485 / 100μlE20-72692-Cy5$485 / 100μlE20-72692-Cy5.5$485 / 100μlE20-72692-Cy7$485 / 100μlE20-72692-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
Categories by research area
Categories by product type
- Antibodies
- Tag antibodies
- Internal control antibodies
- Pathological (Immunohistochemistry, IHC) antibodies
- Cell Signal Transduction Research Antibodies
- Secondary Antibody
- Antibody Sampler Kits
- Recombinant Proteins
- Fluorescence Reagents
- Signal Inhibitors and Activators
- Transfection Reagent
- Cell biology kits