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Mdm2 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72690

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human MDM2. AA range:391-440

  • Source:

    Polyclonal, Rabbit

  • Gene Name:

    MDM2

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

  • Western blot analysis of lysates from SKOV3 cells, using MDM2 Antibody. The lane on the right is blocked with the synthesized peptide.

  • Background:

    MDM2 proto-oncogene(MDM2) Homo sapiens This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013],

  • Protein Name:

    E3 ubiquitin-protein ligase Mdm2

  • Human Gene Id:

    4193

  • Human Swiss Prot No:

    Q00987

  • Mouse Gene Id:

    17246

  • Mouse Swiss Prot No:

    P23804

  • Specificity:

    Mdm2 Polyclonal Antibody detects endogenous levels of Mdm2 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    MDM2; E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2

  • MolecularWeight(Da):

    55233

  • Observed Band(KD):

    90

  • Function:

    disease:Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Four of the splice variants show loss of p53 binding.,domain:Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73 and E2F1. Region II contains most of a central acidic region required for interaction with ribosomal protein L5 and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc interacts specifically with RNA whether or not zinc is present and mediates the heterooligomerization with MDM4. It is also essential for its ubiquitin ligase E3 activity toward p53 and itself.,function:Inhibits TP53/p53- and TP73/p73-mediated cell cycle arrest

  • Subcellular Location:

    nucleus,nucleoplasm,nucleolus,cytoplasm,cytosol,plasma membrane,nuclear body,endocytic vesicle membrane,protein complex,synapse,

  • Expression:

    Blood,Colon,Intestine,Kidney,Lung,Lymphocytes,Malignant fibrous histiocytoma,Neurog

  • Immunofluorescence analysis of A549 cells, using MDM2 Antibody. The picture on the right is blocked with the synthesized peptide.

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Catalog Number

  • Amount:100ul
  • Price:225$

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Mdm2 Polyclonal Antibody | EnoGene Biotech Co. Ltd