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Malic enzyme 1 Polyclonal Antibody
Datasheet
Catalog Number:
E20-72629Amount:
100ulPrice:
225$Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human ME1. AA range:468-517Source:
Polyclonal, RabbitGene Name:
ME1Dilution:
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
- Western blot analysis of lysates from HeLa cells, using ME1 Antibody. The lane on the right is blocked with the synthesized peptide.
Background:
malic enzyme 1(ME1) Homo sapiens This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq, Jul 2008],Protein Name:
NADP-dependent malic enzymeHuman Gene Id:
4199Human Swiss Prot No:
P48163Mouse Gene Id:
17436Mouse Swiss Prot No:
P06801Rat Swiss Prot No:
P13697Specificity:
Malic enzyme 1 Polyclonal Antibody detects endogenous levels of Malic enzyme 1 protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
ME1; NADP-dependent malic enzyme; NADP-ME; Malic enzyme 1MolecularWeight(Da):
64150Observed Band(KD):
64Function:
catalytic activity:(S)-malate + NADP(+) = pyruvate + CO(2) + NADPH.,cofactor:Divalent metal cations. Prefers magnesium or manganese.,online information:The Singapore human mutation and polymorphism database,similarity:Belongs to the malic enzymes family.,subunit:Homotetramer.,tissue specificity:Expressed in all tissues tested including liver, placenta and white adipose tissue.,Subcellular Location:
mitochondrion,cytosol,plasma membrane,Expression:
Liver,Skeletal muscle,Skin,White adipose tissue,
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