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LYPLA1 Polyclonal Antibody
Datasheet
Catalog Number:
E20-72609Amount:
100ulMolecular Weight:
24670 DaObserved Band:25 KDApplications:
WB;IHC-p;IF/ICC;ELISAReactivity:
Human;Rat;Mouse;Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human LYPLA1. AA range:51-100Source:
Polyclonal, Rabbit,IgG
- Western Blot analysis of various cells using LYPLA1 Polyclonal Antibody diluted at 1:2000
Gene Name:
LYPLA1Dilution:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.Protein Name:
Acyl-protein thioesterase 1Human Gene Id:
10434Human Swiss Prot No:
O75608Mouse Gene Id:
18777Mouse Swiss Prot No:
P97823Rat Gene Id:
25514Rat Swiss Prot No:
P70470Specificity:
LYPLA1 Polyclonal Antibody detects endogenous levels of LYPLA1 protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
LYPLA1; APT1; LPL1; Acyl-protein thioesterase 1; APT-1; hAPT1; Lysophospholipase 1; Lysophospholipase I; LPL-I; LysoPLA IBackground:
This gene encodes a member of the alpha/beta hydrolase superfamily. The encoded protein functions as a homodimer, exhibiting both depalmitoylating as well as lysophospholipase activity, and may be involved in Ras localization and signaling. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 4, 6, and 7. [provided by RefSeq, Jul 2013],Function:
catalytic activity:Palmitoyl-protein + H(2)O = palmitate + protein.,function:Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Also has low lysophospholipase activity.,similarity:Belongs to the AB hydrolase 2 family.,subunit:Homodimer.,Subcellular Location:
Cytoplasm . Cell membrane . Nucleus membrane . Endoplasmic reticulum . Shows predominantly a cytoplasmic localization with a weak expression in the cell membrane, nuclear membrane and endoplasmic reticulum. .Expression:
Platelets.
- Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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