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LPAAT-ε Polyclonal Antibody
Datasheet
Catalog Number:
E20-72583Amount:
100ulPrice:
225$Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human AGPAT5. AA range:241-290Source:
Polyclonal, RabbitGene Name:
AGPAT5Dilution:
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
- Western blot analysis of lysates from LOVO cells, using AGPAT5 Antibody. The lane on the right is blocked with the synthesized peptide.
Background:
1-acylglycerol-3-phosphate O-acyltransferase 5(AGPAT5) Homo sapiens This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. This integral membrane protein converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. A pseudogene of this gene is present on the Y chromosome. [provided by RefSeq, Aug 2014],Protein Name:
1-acyl-sn-glycerol-3-phosphate acyltransferase epsilonHuman Gene Id:
55326Human Swiss Prot No:
Q9NUQ2Mouse Swiss Prot No:
Q9D1E8Specificity:
LPAAT-ε Polyclonal Antibody detects endogenous levels of LPAAT-ε protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
AGPAT5; 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon; 1-acylglycerol-3-phosphate O-acyltransferase 5; 1-AGP acyltransferase 5; 1-AGPAT 5; Lysophosphatidic acid acyltransferase epsilon; LPAAT-epsilonMolecularWeight(Da):
42072Observed Band(KD):
45Function:
catalytic activity:Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.,caution:It is uncertain whether Met-1 or Met-12 is the initiator.,domain:The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.,function:Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.,pathway:Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.,similarity:Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.,Subcellular Location:
nuclear envelope,mitochondrion,mitochondrial outer membrane,endoplasmic reticulum membrane,integral component of membrane,Expression:
Amygdala,Brain,Lung,Placenta,
- Western blot analysis of the lysates from HeLa cells using AGPAT5 antibody.
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Catalog Number
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