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LIMK-1 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72562

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human LIMK1. AA range:471-520

  • Source:

    Polyclonal, Rabbit

  • Gao, Ting-Ting, et al. "LIMK1/2 in the mPFC plays a role in chronic stress-induced depressive-like effects in mice." International Journal of Neuropsychopharmacology 23.12 (2020): 821-836.

  • Gene Name:

    LIMK1

  • Dilution:

    Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

  • Background:

    LIM domain kinase 1(LIMK1) Homo sapiens There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cog

  • Protein Name:

    LIM domain kinase 1

  • Human Gene Id:

    3984

  • Human Swiss Prot No:

    P53667

  • Mouse Gene Id:

    16885

  • Mouse Swiss Prot No:

    P53668

  • Rat Swiss Prot No:

    P53669

  • Specificity:

    LIMK-1 Polyclonal Antibody detects endogenous levels of LIMK-1 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    LIMK1; LIMK; LIM domain kinase 1; LIMK-1

  • MolecularWeight(Da):

    72232

  • Observed Band(KD):

    65

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Haploinsufficiency of LIMK1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23.,function:Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development.,PTM:Autophosphorylated.,PTM:Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 PDZ (DHR) doma

  • Subcellular Location:

    nucleoplasm,cytoplasm,cytosol,focal adhesion,membrane,neuron projection,

  • Expression:

    Brain,Epidermal carcinoma,Hippocampus,Placenta,

  • Western blot analysis of lysates from NIH/3T3 cells, treated with UV, using LIMK1 Antibody. The lane on the right is blocked with the synthesized peptide.

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Catalog Number

  • Amount:100ul
  • Price:225$

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