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Laminin β-1 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72528
  • Amount:

    100ul
  • Molecular Weight:

    198066 DaObserved Band:220 KD
  • Applications:

    WB;IHC-p;IF/ICC;ELISA
  • Reactivity:

    Human;Mouse;
  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human LAMB1. AA range:1721-1770
  • Source:

    Polyclonal, Rabbit,IgG
  • Immunofluorescence analysis of Hela cell. 1,Laminin β-1 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Kif 7 Monoclonal Antibody(3F8)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
  • Gene Name:

    LAMB1
  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
  • Protein Name:

    Laminin subunit beta-1
  • Human Gene Id:

    3912
  • Human Swiss Prot No:

    P07942
  • Mouse Swiss Prot No:

    P02469
  • Specificity:

    Laminin β-1 Polyclonal Antibody detects endogenous levels of Laminin β-1 protein.
  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Concentration:

    1 mg/ml
  • Storage Stability:

    -20°C/1 year
  • Other Name:

    LAMB1; Laminin subunit beta-1; Laminin B1 chain; Laminin-1 subunit beta; Laminin-10 subunit beta; Laminin-12 subunit beta; Laminin-2 subunit beta; Laminin-6 subunit beta; Laminin-8 subunit beta
  • Background:

    Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biol
  • Function:

    domain:Domains VI and IV are globular.,domain:The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.,function:Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.,similarity:Contains 1 laminin IV type B domain.,similarity:Contains 1 laminin N-terminal domain.,similarity:Contains 13 laminin EGF-like domains.,subcellular location:Major component.,subunit:Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Beta-1 is a subunit of laminin-1 (EHS laminin), lamin
  • Subcellular Location:

    Secreted, extracellular space, extracellular matrix, basement membrane. Major component.
  • Expression:

    Colon,Liver,Muscle,Plasma,
  • Western Blot analysis of various cells using Laminin β-1 Polyclonal Antibody diluted at 1:1000

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Catalog Number

  • Amount:100ul
  • Price:225$

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