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KEPI Polyclonal Antibody
Datasheet
Catalog Number:
E20-72464Amount:
100ulMolecular Weight:
17843DaApplications:
WB;IHC-p;IF(paraffin section);ELISAReactivity:
Human;MousePurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human PPP1R14C. AA range:51-100Source:
Polyclonal, Rabbit,IgG
- Western Blot analysis of various cells using KEPI Polyclonal Antibody diluted at 1:1000
Gene Name:
PPP1R14CDilution:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.Protein Name:
Protein phosphatase 1 regulatory subunit 14CHuman Gene Id:
81706Human Swiss Prot No:
Q8TAE6Mouse Gene Id:
76142Mouse Swiss Prot No:
Q8R4S0Rat Gene Id:
171010Rat Swiss Prot No:
Q8R4R9Specificity:
KEPI Polyclonal Antibody detects endogenous levels of KEPI protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
PPP1R14C; KEPI; Protein phosphatase 1 regulatory subunit 14C; Kinase-enhanced PP1 inhibitor; PKC-potentiated PP1 inhibitory protein; Serologically defined breast cancer antigen NY-BR-81Observed Band(KD):
20Background:
The degree of protein phosphorylation is regulated by a balance of protein kinase and phosphatase activities. Protein phosphatase-1 (PP1; see MIM 176875) is a signal-transducing phosphatase that influences neuronal activity, protein synthesis, metabolism, muscle contraction, and cell division. PPP1R14C is an inhibitor of PP1 (Liu et al., 2002 [PubMed 11812771]).[supplied by OMIM, Feb 2010],Function:
function:Inhibitor of PPP1CA. Has over 600-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.,similarity:Belongs to the PP1 inhibitor family.,tissue specificity:Detected in breast cancer.,Subcellular Location:
Cytoplasm . Membrane ; Peripheral membrane protein .Expression:
Detected in breast cancer.
- Western blot analysis of lysates from Jurkat and COLO205 cells, using PPP1R14C Antibody. The lane on the right is blocked with the synthesized peptide.
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