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IRF-3 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72398

  • Amount:

    100ul

  • Molecular Weight:

    47219Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human IRF3. AA range:362-411

  • Source:

    Polyclonal, Rabbit,IgG

  • Jiang, Z., Wei, F., Zhang, Y. et al. IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection. Nat Microbiol 6, 932–945 (2021).

  • Gene Name:

    IRF3

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

  • Protein Name:

    Interferon regulatory factor 3

  • Human Gene Id:

    3661

  • Human Swiss Prot No:

    Q14653

  • Mouse Gene Id:

    54131

  • Mouse Swiss Prot No:

    P70671

  • Specificity:

    IRF-3 Polyclonal Antibody detects endogenous levels of IRF-3 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    IRF3; Interferon regulatory factor 3; IRF-3

  • Observed Band(KD):

    48-55kd

  • Background:

    This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011],

  • Function:

    function:Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.,PTM:Constitutively phosphorylated on many serines residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Ser-385 and Ser-386 may be specifically phosphoryla

  • Subcellular Location:

    Cytoplasm . Nucleus . Mitochondrion . Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect (PubMed:10805757). When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm (PubMed:10805757). Recruited to mitochondria via TOMM70:HSP90AA1 upon Sendai virus infection (PubMed:25609812). .

  • Expression:

    Expressed constitutively in a variety of tissues.

  • Western Blot analysis of various cells using IRF-3 Polyclonal Antibody diluted at 1:500

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Catalog Number

  • Amount:100ul
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IRF-3 Polyclonal Antibody | EnoGene Biotech Co. Ltd