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IRAK-1 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72391

  • Amount:

    100ul

  • Molecular Weight:

    76537Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human IRAK1. AA range:353-402

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using IRAK-1 Polyclonal Antibody

  • Gene Name:

    IRAK1

  • Dilution:

    WB 1:500-2000;IHC-p 1:50-300

  • Protein Name:

    Interleukin-1 receptor-associated kinase 1

  • Human Gene Id:

    3654

  • Human Swiss Prot No:

    P51617

  • Mouse Gene Id:

    16179

  • Mouse Swiss Prot No:

    Q62406

  • Specificity:

    IRAK-1 Polyclonal Antibody detects endogenous levels of IRAK-1 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    IRAK1; IRAK; Interleukin-1 receptor-associated kinase 1; IRAK-1

  • Observed Band(KD):

    76

  • Background:

    This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.,PTM:Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.,similarity:

  • Subcellular Location:

    Cytoplasm . Nucleus . Lipid droplet . Translocates to the nucleus when sumoylated. RSAD2/viperin recruits it to the lipid droplet (By similarity). .

  • Expression:

    Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.

  • Western blot analysis of lysates from HeLa cells, using IRAK1 Antibody. The lane on the right is blocked with the synthesized peptide.

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