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Cell Signal Transduction Research Antibodies
IKKα/β Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight84654/86564DaObserved Band:84KD
Protein NameInhibitor of nuclear factor kappa-B kinase subunit alpha
Human Gene Id1147/3551
Human Swiss Prot NoO15111/O14920
Mouse Gene Id16150
Rat Gene Id84351
Rat Swiss Prot NoQ9QY78
SpecificityIKKα/β Polyclonal Antibody detects endogenous levels of IKKα/β protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameCHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B
Functioncatalytic activity:ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].,enzyme regulation:Activated when phosphorylated and inactivated when dephosphorylated.,function:Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.,PTM:Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK
Subcellular Locationintracellular,nucleoplasm,cytoplasm,cytosol,IkappaB kinase complex,cytoplasmic side of plasma membrane,CD40 receptor complex,intracellular membrane-bounded organelle,
ExpressionBrain,Cervix carcinoma,Heart,Lymph,T-cell,,
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from human IKK-alpha/beta. AA range:151-200
Gene NameCHUK/IKBKB
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Backgroundconserved helix-loop-helix ubiquitous kinase(CHUK) Homo sapiens This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008],
Conjugated Antibody
This product is also available conjugated. Format: E20-72301-Conjugate. Price: $485 / Size: 100μl.
E20-72301-FITC$485 / 100μlE20-72301-Biotin$485 / 100μlE20-72301-HRP$485 / 100μlE20-72301-AP$485 / 100μlE20-72301-AF350$485 / 100μlE20-72301-AF405M$485 / 100μlE20-72301-AF488$485 / 100μlE20-72301-AF514$485 / 100μlE20-72301-AF532$485 / 100μlE20-72301-AF555$485 / 100μlE20-72301-AF568$485 / 100μlE20-72301-AF405S$485 / 100μlE20-72301-AF405L$485 / 100μlE20-72301-AF546$485 / 100μlE20-72301-AF594$485 / 100μlE20-72301-AF610$485 / 100μlE20-72301-AF635$485 / 100μlE20-72301-AF647$485 / 100μlE20-72301-AF680$485 / 100μlE20-72301-AF700$485 / 100μlE20-72301-AF750$485 / 100μlE20-72301-AF790$485 / 100μlE20-72301-APC$485 / 100μlE20-72301-PE$485 / 100μlE20-72301-Cy3$485 / 100μlE20-72301-Cy5$485 / 100μlE20-72301-Cy5.5$485 / 100μlE20-72301-Cy7$485 / 100μlE20-72301-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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