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IARS2 Polyclonal Antibody
Datasheet
Catalog Number:
E20-72267Amount:
100ulMolecular Weight:
113792DaApplications:
WB;IHC-p;IF(paraffin section);ELISAReactivity:
Human;MousePurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human IARS2. AA range:71-120Source:
Polyclonal, Rabbit,IgG
- Western Blot analysis of various cells using IARS2 Polyclonal Antibody diluted at 1:2000
Gene Name:
IARS2Dilution:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.Protein Name:
Isoleucine--tRNA ligase mitochondrialHuman Gene Id:
55699Human Swiss Prot No:
Q9NSE4Mouse Gene Id:
381314Mouse Swiss Prot No:
Q8BIJ6Specificity:
IARS2 Polyclonal Antibody detects endogenous levels of IARS2 protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
IARS2; Isoleucine--tRNA ligase; mitochondrial; Isoleucyl-tRNA synthetase; IleRSObserved Band(KD):
114Background:
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Dec 2014],Function:
catalytic activity:ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).,similarity:Belongs to the class-I aminoacyl-tRNA synthetase family.,Subcellular Location:
Mitochondrion matrix .Expression:
Brain,Epithelium,Liver,Placenta,Skin,.
- Western Blot analysis of NIH-3T3 cells using IARS2 Polyclonal Antibody diluted at 1:2000
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