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HXK I Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72265

  • Amount:

    100ul

  • Molecular Weight:

    102486Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human HXK1. AA range:31-80

  • Source:

    Polyclonal, Rabbit,IgG

  • Gao, Yunshu, et al. "Overexpression of metabolic markers HK1 and PKM2 contributes to lymphatic metastasis and adverse prognosis in Chinese gastric cancer." International journal of clinical and experimental pathology 8.8 (2015): 9264.

  • Gene Name:

    HK1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

  • Protein Name:

    Hexokinase-1

  • Human Gene Id:

    3098

  • Human Swiss Prot No:

    P19367

  • Mouse Swiss Prot No:

    P17710

  • Rat Gene Id:

    25058

  • Rat Swiss Prot No:

    P05708

  • Specificity:

    HXK I Polyclonal Antibody detects endogenous levels of HXK I protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    HK1; Hexokinase-1; Brain form hexokinase; Hexokinase type I; HK I

  • Observed Band(KD):

    109

  • Background:

    Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016],

  • Function:

    catalytic activity:ATP + D-hexose = ADP + D-hexose 6-phosphate.,disease:Defects in HK1 are the cause of hexokinase deficiency [MIM:235700]. Hexokinase deficiency is a rare autosomal recessive disease with nonspherocytic hemolytic anemia as the predominant clinical feature.,domain:The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.,enzyme regulation:Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.,miscellaneous:In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).,online information:Hexokinase entry,pathway:Carbohydrate metabolism; hexose metabolism.,similarity:Belongs to the hexokinase family.,subcellular location:Its hydrophobic N-ter

  • Subcellular Location:

    Mitochondrion outer membrane ; Peripheral membrane protein . Cytoplasm, cytosol . The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane (Probable). Dissociates from the mitochondrial outer membrane following inhibition by N-acetyl-D-glucosamine, leading to relocation to the cytosol (PubMed:27374331). .

  • Expression:

    Isoform 2: Erythrocyte specific (Ref.6). Isoform 3: Testis-specific (PubMed:10978502). Isoform 4: Testis-specific (PubMed:10978502)..

  • Western blot analysis of HXK I Polyclonal Antibody, using Hela, MCF7,SW480,293T cell, 4° over night, secondary antibody(cat: RS0002 was diluted at 1:10000, 37° 1hour.

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Catalog Number

  • Amount:100ul
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HXK I Polyclonal Antibody | EnoGene Biotech Co. Ltd