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HSP40 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72254

  • Amount:

    100ul

  • Molecular Weight:

    38044Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human HSP40. AA range:271-320

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using HSP40 Polyclonal Antibody

  • Gene Name:

    DNAJB1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

  • Protein Name:

    DnaJ homolog subfamily B member 1

  • Human Gene Id:

    3337

  • Human Swiss Prot No:

    P25685

  • Mouse Gene Id:

    81489

  • Mouse Swiss Prot No:

    Q9QYJ3

  • Specificity:

    HSP40 Polyclonal Antibody detects endogenous levels of HSP40 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    DNAJB1; DNAJ1; HDJ1; HSPF1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; Heat shock 40 kDa protein 1; HSP40; Heat shock protein 40; Human DnaJ protein 1; hDj-1

  • Observed Band(KD):

    45

  • Background:

    This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015],

  • Function:

    function:Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.,induction:By heat shock.,similarity:Contains 1 J domain.,subcellular location:Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.,subunit:Interacts with DNAJC3.,

  • Subcellular Location:

    Cytoplasm . Nucleus . Nucleus, nucleolus . Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.

  • Expression:

    Brain,Heart,Lung,Placenta,.

  • Western blot analysis of lysates from COLO205 cells, using HSP40 Antibody. The lane on the right is blocked with the synthesized peptide.

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