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HSF-27 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72238
  • Amount:

    100ul
  • Molecular Weight:

    56921Da
  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA
  • Reactivity:

    Human;Mouse
  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human CDK5RAP3. AA range:341-390
  • Source:

    Polyclonal, Rabbit,IgG
  • Western Blot analysis of various cells using HSF-27 Polyclonal Antibody diluted at 1:2000
  • Gene Name:

    CDK5RAP3
  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
  • Protein Name:

    CDK5 regulatory subunit-associated protein 3
  • Human Gene Id:

    80279
  • Human Swiss Prot No:

    Q96JB5
  • Mouse Gene Id:

    80280
  • Mouse Swiss Prot No:

    Q99LM2
  • Rat Gene Id:

    80278
  • Rat Swiss Prot No:

    Q9JLH7
  • Specificity:

    HSF-27 Polyclonal Antibody detects endogenous levels of HSF-27 protein.
  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Concentration:

    1 mg/ml
  • Storage Stability:

    -20°C/1 year
  • Other Name:

    CDK5RAP3; IC53; MSTP016; OK/SW-cl.114; PP1553; CDK5 regulatory subunit-associated protein 3; CDK5 activator-binding protein C53; Protein HSF-27
  • Observed Band(KD):

    60
  • Background:

    This gene encodes a protein that has been reported to function in signaling pathways governing transcriptional regulation and cell cycle progression. It may play a role in tumorigenesis and metastasis. A pseudogene of this gene is located on the long arm of chromosome 20. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, May 2013],
  • Function:

    function:Potential regulator of CDK5 activity. May be involved in cell proliferation. Regulates CDK5 activity via its interaction with CDK5R1.,PTM:Phosphorylated in vitro by CDK5.,similarity:Belongs to the CDK5RAP3 family.,subunit:Interacts with CDK5R1. This interaction is prevented by the association between CDK5R1 and CDK5RAP3 (By similarity). Interacts with the enteric adenovirus serotype 41 fiber protein.,tissue specificity:Ubiquitous. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta.,
  • Subcellular Location:

    Nucleus . Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Colocalizes and associates with microtubules. .
  • Expression:

    Ubiquitously expressed (PubMed:12054757, PubMed:10721722). Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta (PubMed:12737517)..
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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Catalog Number

  • Amount:100ul
  • Price:225$

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HSF-27 Polyclonal Antibody | EnoGene Biotech Co. Ltd