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HSF1 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72236

  • Amount:

    100ul

  • Molecular Weight:

    30603Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human HSF1. AA range:270-319

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using HSF1 Polyclonal Antibody diluted at 1:1000

  • Gene Name:

    HSF1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

  • Protein Name:

    Heat shock factor protein 1

  • Human Gene Id:

    3297

  • Human Swiss Prot No:

    Q00613

  • Mouse Gene Id:

    15499

  • Mouse Swiss Prot No:

    P38532

  • Specificity:

    HSF1 Polyclonal Antibody detects endogenous levels of HSF1 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    HSF1; HSTF1; Heat shock factor protein 1; HSF 1; Heat shock transcription factor 1; HSTF 1

  • Observed Band(KD):

    82

  • Background:

    heat shock transcription factor 1(HSF1) Homo sapiens The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phsphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Aug 2016],

  • Function:

    function:DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.,PTM:Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transacti

  • Subcellular Location:

    Nucleus . Cytoplasm . Nucleus, nucleoplasm . Cytoplasm, perinuclear region . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Chromosome, centromere, kinetochore . The monomeric form is cytoplasmic in unstressed cells (PubMed:8455624, PubMed:26159920). Predominantly nuclear protein in both unstressed and heat shocked cells (PubMed:10413683, PubMed:10359787). Translocates in the nucleus upon heat shock (PubMed:8455624). Nucleocytoplasmic shuttling protein (PubMed:26159920). Colocalizes with IER5 in the nucleus (PubMed:27354066). Colocalizes with BAG3 to the nucleus upon heat stress (PubMed:8455624, PubMed:26159920). Localizes in subnuclear granules called nuclear stress bodies (nSBs) upon heat shock (PubMed:11447121, PubMed:1151455

  • Expression:

    Adipose tissue,Brain,Epithelium,Muscle,

  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HSF1 Antibody. The picture on the right is blocked with the synthesized peptide.

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Catalog Number

  • Amount:100ul
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HSF1 Polyclonal Antibody | EnoGene Biotech Co. Ltd