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hnRNP G Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72200

  • Amount:

    100ul

  • Molecular Weight:

    42332Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human hnRNP G. AA range:6-55

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using hnRNP G Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

  • Gene Name:

    RBMX

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

  • Protein Name:

    RNA-binding motif protein X chromosome

  • Human Gene Id:

    27316

  • Human Swiss Prot No:

    P38159

  • Mouse Gene Id:

    19655

  • Mouse Swiss Prot No:

    Q9WV02

  • Rat Gene Id:

    302855

  • Rat Swiss Prot No:

    Q4V898

  • Specificity:

    hnRNP G Polyclonal Antibody detects endogenous levels of hnRNP G protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    RBMX; HNRPG; RBMXP1; RNA-binding motif protein; X chromosome; Glycoprotein p43; Heterogeneous nuclear ribonucleoprotein G; hnRNP G

  • Observed Band(KD):

    40

  • Background:

    This gene belongs to the RBMY gene family which includes candidate Y chromosome spermatogenesis genes. This gene, an active X chromosome homolog of the Y chromosome RBMY gene, is widely expressed whereas the RBMY gene evolved a male-specific function in spermatogenesis. Pseudogenes of this gene, found on chromosomes 1, 4, 9, 11, and 6, were likely derived by retrotransposition from the original gene. Alternatively spliced transcript variants encoding different isoforms have been identified. A snoRNA gene (SNORD61) is found in one of its introns. [provided by RefSeq, Sep 2009],

  • Function:

    function:RNA-binding protein which may be involved in pre-mRNA splicing.,PTM:Arg-185 is dimethylated, probably to asymmetric dimethylarginine.,PTM:Cleavage of initiator Met is partial. If Met-1 is not removed, it is acetylated. If it is removed, Val-2 is acetylated.,PTM:O-glycosylated.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subcellular location:Component of ribonucleosomes.,subunit:Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2

  • Subcellular Location:

    Nucleus . Component of ribonucleosomes. Localizes in numerous small granules in the nucleus.

  • Expression:

    Expressed strongly in oral keratinocytes, but only weakly detected in oral squamous cell carcinomas (at protein level).

  • Immunofluorescence analysis of HepG2 cells, using hnRNP G Antibody. The picture on the right is blocked with the synthesized peptide.

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