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hnRNP F Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72199

  • Amount:

    100ul

  • Molecular Weight:

    45672Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human hnRNP F. AA range:11-60

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of HepG2 cells using hnRNP F Polyclonal Antibody

  • Gene Name:

    HNRNPF

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

  • Protein Name:

    Heterogeneous nuclear ribonucleoprotein F

  • Human Gene Id:

    3185

  • Human Swiss Prot No:

    P52597

  • Mouse Gene Id:

    98758

  • Mouse Swiss Prot No:

    Q9Z2X1

  • Rat Gene Id:

    64200

  • Rat Swiss Prot No:

    Q794E4

  • Specificity:

    hnRNP F Polyclonal Antibody detects endogenous levels of hnRNP F protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    HNRNPF; HNRPF; Heterogeneous nuclear ribonucleoprotein F; hnRNP F; Nucleolin-like protein mcs94-1

  • Observed Band(KD):

    48

  • Background:

    This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008],

  • Function:

    function:Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Probably binds G-rich sequences in pre-mRNAs.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 3 RRM (RNA recognition motif) domains.,subunit:Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRNPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SN

  • Subcellular Location:

    Nucleus, nucleoplasm.

  • Expression:

    Expressed ubiquitously.

  • Western blot analysis of lysates from HepG2 cells, using hnRNP F Antibody. The lane on the right is blocked with the synthesized peptide.

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Catalog Number

  • Amount:100ul
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