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HMG-1 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72183

  • Amount:

    100ul

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human HMGB1. AA range:131-180

  • Source:

    Polyclonal, Rabbit,IgG

  • Immunofluorescence analysis of Hela cell. 1,HMG-1 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). p53 Monoclonal Antibody(6C4)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).

  • Gene Name:

    HMGB1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

  • Protein Name:

    High mobility group protein B1

  • Human Gene Id:

    3146

  • Human Swiss Prot No:

    P09429

  • Mouse Gene Id:

    1.00862e+008

  • Mouse Swiss Prot No:

    P63158

  • Rat Gene Id:

    25459

  • Rat Swiss Prot No:

    P63159

  • Specificity:

    HMG-1 Polyclonal Antibody detects endogenous levels of HMG-1 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    HMGB1; HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1

  • Observed Band(KD):

    about 30kd

  • Background:

    This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015],

  • Function:

    function:Binds preferentially single-stranded DNA and unwinds double stranded DNA.,similarity:Belongs to the HMGB family.,similarity:Contains 2 HMG box DNA-binding domains.,

  • Subcellular Location:

    Nucleus . Chromosome . Cytoplasm . Secreted . Cell membrane ; Peripheral membrane protein ; Extracellular side . Endosome . Endoplasmic reticulum-Golgi intermediate compartment . In basal state predominantly nuclear. Shuttles between the cytoplasm and the nucleus (PubMed:12231511, PubMed:17114460). Translocates from the nucleus to the cytoplasm upon autophagy stimulation (PubMed:20819940). Release from macrophages in the extracellular milieu requires the activation of NLRC4 or NLRP3 inflammasomes (By similarity). Passively released to the extracellular milieu from necrotic cells by diffusion, involving the fully reduced HGMB1 which subsequently gets oxidized (PubMed:19811284). Also released from apoptotic cells (PubMed:16855214, PubMed:18631454). Active secretion from a variety of immune a

  • Expression:

    Ubiquitous. Expressed in platelets (PubMed:11154118).

  • Western Blot analysis of various cells using HMG-1 Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

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Catalog Number

  • Amount:100ul
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