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Histone H2A.X Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72154

  • Amount:

    100ul

  • Molecular Weight:

    15145Da

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Rat;Mouse;

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Histone H2AX . AA range:94-143

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Histone H2A.X Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

  • Gene Name:

    H2AFX

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

  • Protein Name:

    Histone H2A.x

  • Human Gene Id:

    3014

  • Human Swiss Prot No:

    P16104

  • Mouse Swiss Prot No:

    P27661

  • Specificity:

    Histone H2A.X Polyclonal Antibody detects endogenous levels of Histone H2A.X protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    H2AFX; H2AX; Histone H2A.x; H2a/x

  • Observed Band(KD):

    15

  • Background:

    H2A histone family member X(H2AFX) Homo sapiens Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015],

  • Function:

    developmental stage:Synthesized in G1 as well as in S-phase.,domain:The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.,function:Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.,PTM:Mon

  • Subcellular Location:

    Nucleus . Chromosome .

  • Expression:

    Lung,Placenta,

  • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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