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HGK Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72130

  • Amount:

    100ul

  • Molecular Weight:

    ： 142101Da

  • Applications:

    WB;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse

  • Purification:

    ： The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    ： The antiserum was produced against synthesized peptide derived from human MEKKK 4. AA range:406-455

  • Source:

    ： Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using HGK Polyclonal Antibody

  • Gene Name:

    ： MAP4K4

  • Dilution:

    ： Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

  • Protein Name:

    ： Mitogen-activated protein kinase kinase kinase kinase 4

  • Human Gene Id:

    ： 9448

  • Human Swiss Prot No:

    ： O95819

  • Mouse Gene Id:

    ： 26921

  • Mouse Swiss Prot No:

    ： P97820

  • Specificity:

    ： HGK Polyclonal Antibody detects endogenous levels of HGK protein.

  • Formulation:

    ： Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    ： 1 mg/ml

  • Storage Stability:

    ： -20°C/1 year

  • Other Name:

    ： MAP4K4; HGK; KIAA0687; NIK; Mitogen-activated protein kinase kinase kinase kinase 4; HPK/GCK-like kinase HGK; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; Nck-interacting kinase

  • Observed Band(KD):

    ： 142

  • Background:

    ： mitogen-activated protein kinase kinase kinase kinase 4(MAP4K4) Homo sapiens The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],

  • Function:

    ： catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.,similarity:Contains 1 CNH domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with the SH3 domain of the adapter proteins Nck (By similarity). Binds, via its CNH regulatory domain, to the N-terminal region of SPG3A.,tissue specificity:Appears to be ubiquitous, expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain, isoform 4 is predominant in the liver, skelet

  • Subcellular Location:

    ： Cytoplasm .

  • Expression:

    ： Widely expressed. Isoform 5 is abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.

  • Immunofluorescence analysis of A549 cells, using MEKKK 4 Antibody. The picture on the right is blocked with the synthesized peptide.

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Catalog Number

  • Amount:100ul
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HGK Polyclonal Antibody | EnoGene Biotech Co. Ltd