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HES-6 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72127

  • Amount:

    100ul

  • Molecular Weight:

    24129Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Rat;Mouse;

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human HES6. AA range:1-50

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using HES-6 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

  • Gene Name:

    HES6

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

  • Protein Name:

    Transcription cofactor HES-6

  • Human Gene Id:

    55502

  • Human Swiss Prot No:

    Q96HZ4

  • Mouse Swiss Prot No:

    Q9JHE6

  • Specificity:

    HES-6 Polyclonal Antibody detects endogenous levels of HES-6 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    HES6; BHLHB41; Transcription cofactor HES-6; C-HAIRY1; Class B basic helix-loop-helix protein 41; bHLHb41; Hairy and enhancer of split 6

  • Observed Band(KD):

    30

  • Background:

    hes family bHLH transcription factor 6(HES6) Homo sapiens This gene encodes a member of a subfamily of basic helix-loop-helix transcription repressors that have homology to the Drosophila enhancer of split genes. Members of this gene family regulate cell differentiation in numerous cell types. The protein encoded by this gene functions as a cofactor, interacting with other transcription factors through a tetrapeptide domain in its C-terminus. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Dec 2008],

  • Function:

    domain:Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).,domain:The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.,function:Does not bind DNA itself but suppresses both HES1-mediated N box-dependent transcriptional repression and binding of HES1 to E box sequences. Also suppresses HES1-mediated inhibition of the heterodimer formed by ASCL1/MASH1 and TCF3/E47, allowing ASCL1 and TCF3 to up-regulate transcription in its presence. Promotes cell differentation.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,similarity:Contains 1 Orange domain.,subunit:Transcription repression requires formation of a complex wit

  • Subcellular Location:

    Nucleus .

  • Expression:

    Muscle,Ovary,

  • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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Catalog Number

  • Amount:100ul
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HES-6 Polyclonal Antibody | EnoGene Biotech Co. Ltd