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HEI10 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-72122

  • Amount:

    100ul

  • Molecular Weight:

    31544Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human CCNB1IP1. AA range:201-250

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using HEI10 Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

  • Gene Name:

    CCNB1IP1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

  • Protein Name:

    E3 ubiquitin-protein ligase CCNB1IP1

  • Human Gene Id:

    57820

  • Human Swiss Prot No:

    Q9NPC3

  • Specificity:

    HEI10 Polyclonal Antibody detects endogenous levels of HEI10 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CCNB1IP1; C14orf18; HEI10; E3 ubiquitin-protein ligase CCNB1IP1; Cyclin-B1-interacting protein 1; Human enhancer of invasion 10

  • Observed Band(KD):

    32

  • Background:

    cyclin B1 interacting protein 1(CCNB1IP1) Homo sapiens HEI10 is a member of the E3 ubiquitin ligase family and functions in progression of the cell cycle through G(2)/M.[supplied by OMIM, Apr 2004],

  • Function:

    function:E3 ubiquitin-protein ligase. Modulates cyclin B levels and participates in the regulation of cell cycle progression through the G2 phase. Overexpression causes delayed entry into mitosis.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated by CDC2 on serine or threonine residues (in vitro).,PTM:Ubiquitinated; autoubiquitinated.,similarity:Contains 1 RING-type zinc finger.,subcellular location:May associate with segregating chromosomes during metaphase and anaphase.,subunit:Binds CCNB1 and UBE2L3.,tissue specificity:Highly expressed in heart. Detected at intermediate levels in liver and kidney, and at low levels in placenta, brain and lung.,

  • Subcellular Location:

    Nucleus. Chromosome. Associates to the synaptonemal complex.

  • Expression:

    Highly expressed in heart. Detected at intermediate levels in liver and kidney, and at low levels in placenta, brain and lung.

  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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