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GRK 3 Polyclonal Antibody
Datasheet
Catalog Number:
E20-72068Amount:
100ulMolecular Weight:
79710DaApplications:
WB;IHC-p;IF(paraffin section);ELISAReactivity:
Human;Rat;Mouse;Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human GRK3. AA range:361-410Source:
Polyclonal, Rabbit,IgG
- Western Blot analysis of various cells using GRK 3 Polyclonal Antibody diluted at 1:500
Gene Name:
ADRBK2Dilution:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.Protein Name:
Beta-adrenergic receptor kinase 2Human Gene Id:
157Human Swiss Prot No:
P35626Mouse Gene Id:
320129Mouse Swiss Prot No:
Q3UYH7Rat Gene Id:
25372Rat Swiss Prot No:
P26819Specificity:
GRK 3 Polyclonal Antibody detects endogenous levels of GRK 3 protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
ADRBK2; BARK2; GRK3; Beta-adrenergic receptor kinase 2; Beta-ARK-2; G-protein-coupled receptor kinase 3Observed Band(KD):
80Background:
The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. [provided by RefSeq, Jul 2008],Function:
catalytic activity:ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.,function:Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RGS domain.,Subcellular Location:
Cell junction, synapse, postsynapse . Cell junction, synapse, presynapse .Expression:
Brain,Ovary,
- Western blot analysis of lysates from HepG2 and HeLa cells, using GRK3 Antibody. The lane on the right is blocked with the synthesized peptide.
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