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Granzyme K Polyclonal Antibody
Datasheet
Catalog Number:
E20-72051Amount:
100ulMolecular Weight:
28882DaApplications:
WB;IHC-p;IF/ICC;ELISAReactivity:
Human;Rat;Mouse;Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human GRAK. AA range:61-110Source:
Polyclonal, Rabbit,IgG
- Western Blot analysis of various cells using Granzyme K Polyclonal Antibody diluted at 1:1000
Gene Name:
GZMKDilution:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.Protein Name:
Granzyme KHuman Gene Id:
3003Human Swiss Prot No:
P49863Mouse Gene Id:
14945Mouse Swiss Prot No:
O35205Rat Gene Id:
29165Rat Swiss Prot No:
P49864Specificity:
Granzyme K Polyclonal Antibody detects endogenous levels of Granzyme K protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
GZMK; TRYP2; Granzyme K; Fragmentin-3; Granzyme-3; NK-tryptase-2; NK-Tryp-2Observed Band(KD):
33Background:
This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. [provided by RefSeq, Jul 2008],Function:
similarity:Belongs to the peptidase S1 family. Granzyme subfamily.,similarity:Contains 1 peptidase S1 domain.,tissue specificity:Expressed in lung, spleen, thymus and peripheral blood leukocytes.,Subcellular Location:
Secreted. Cytoplasmic granule.Expression:
Expressed in lung, spleen, thymus and peripheral blood leukocytes.
- Immunofluorescence analysis of NIH/3T3 cells, using GRAK Antibody. The picture on the right is blocked with the synthesized peptide.
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