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Cell Signal Transduction Research Antibodies
GLCNE Polyclonal Antibody
Human;Mouse;Rat
WB;IHC-p;IF(paraffin section);ELISA
Polyclonal, Rabbit,IgG
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight79275Da
Protein NameBifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
Human Gene Id10020
Human Swiss Prot NoQ9Y223
Mouse Gene Id50798
Mouse Swiss Prot NoQ91WG8
Rat Gene Id114711
Rat Swiss Prot NoO35826
SpecificityGLCNE Polyclonal Antibody detects endogenous levels of GLCNE protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameGNE; GLCNE; Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase
Observed Band(KD)80
Functioncatalytic activity:ATP + N-acyl-D-mannosamine = ADP + N-acyl-D-mannosamine 6-phosphate.,catalytic activity:UDP-N-acetyl-D-glucosamine = UDP-N-acetyl-D-mannosamine.,disease:Defects in GNE are a cause of sialuria [MIM:269921]; also known as sialuria French type. In sialuria, free sialic acid accumulates in the cytoplasm and gram quantities of neuraminic acid are secreted in the urine. The metabolic defect involves lack of feedback inhibition of UDP-GlcNAc 2-epimerase by CMP-Neu5Ac, resulting in constitutive overproduction of free Neu5Ac. Clinical features include variable degrees of developmental delay, coarse facial features and hepatomegaly. Sialuria inheritance is autosomal dominant.,disease:Defects in GNE are the cause of inclusion body myopathy type 2 (IBM2) [MIM:600737]. Hereditary inclusion body myopathies are a group of neuromuscular disorders characterized by adult onset, slowly p
Subcellular LocationCytoplasm .
ExpressionHighest expression in liver and placenta. Also found in heart, brain, lung, kidney, skeletal muscle and pancreas. Isoform 1 is expressed in heart, brain, kidney, liver, placenta, lung, spleen, pancreas, skeletal muscle and colon. Isoform 2 is expressed mainly in placenta, but also in brain, kidney, liver, lung, pancreas and colon. Isoform 3 is expressed at low level in kidney, liver, placenta and colon.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from human GNE. AA range:592-641
Gene NameGNE
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
BackgroundThe protein encoded by this gene is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells. Mutations in this gene are associated with sialuria, autosomal recessive inclusion body myopathy, and Nonaka myopathy. Alternative splicing of this gene results in transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
Conjugated Antibody
This product is also available conjugated. Format: E20-71913-Conjugate. Price: $485 / Size: 100μl.
E20-71913-FITC$485 / 100μlE20-71913-Biotin$485 / 100μlE20-71913-HRP$485 / 100μlE20-71913-AP$485 / 100μlE20-71913-AF350$485 / 100μlE20-71913-AF405M$485 / 100μlE20-71913-AF488$485 / 100μlE20-71913-AF514$485 / 100μlE20-71913-AF532$485 / 100μlE20-71913-AF555$485 / 100μlE20-71913-AF568$485 / 100μlE20-71913-AF405S$485 / 100μlE20-71913-AF405L$485 / 100μlE20-71913-AF546$485 / 100μlE20-71913-AF594$485 / 100μlE20-71913-AF610$485 / 100μlE20-71913-AF635$485 / 100μlE20-71913-AF647$485 / 100μlE20-71913-AF680$485 / 100μlE20-71913-AF700$485 / 100μlE20-71913-AF750$485 / 100μlE20-71913-AF790$485 / 100μlE20-71913-APC$485 / 100μlE20-71913-PE$485 / 100μlE20-71913-Cy3$485 / 100μlE20-71913-Cy5$485 / 100μlE20-71913-Cy5.5$485 / 100μlE20-71913-Cy7$485 / 100μlE20-71913-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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