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GCS-α-1 Polyclonal Antibody
Datasheet
Catalog Number:
E20-71885Amount:
100ulMolecular Weight:
77452DaObserved Band:77KDApplications:
WB;CoIP;IHC-p;IF(paraffin section);ELISAReactivity:
Human;Mouse;RatPurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human GCS-alpha-1. AA range:374-423Source:
Polyclonal, Rabbit,IgG
- Western Blot analysis of various cells using GCS-α-1 Polyclonal Antibody
Gene Name:
GUCY1A3Dilution:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.Protein Name:
Guanylate cyclase soluble subunit alpha-3Human Gene Id:
2982Human Swiss Prot No:
Q02108Mouse Gene Id:
60596Mouse Swiss Prot No:
Q9ERL9Rat Swiss Prot No:
P19686Specificity:
GCS-α-1 Polyclonal Antibody detects endogenous levels of GCS-α-1 protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
GUCY1A3; GUC1A3; GUCSA3; GUCY1A1; Guanylate cyclase soluble subunit alpha-3; GCS-alpha-3; GCS-alpha-1; Soluble guanylate cyclase large subunitBackground:
Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Jan 2012],Function:
catalytic activity:GTP = 3',5'-cyclic GMP + diphosphate.,enzyme regulation:Activated by nitric oxide in the presence of magnesium or manganese ions.,miscellaneous:There are two types of guanylate cyclases: soluble forms and membrane-associated receptor forms.,similarity:Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.,similarity:Contains 1 guanylate cyclase domain.,subunit:Heterodimer of an alpha and a beta chain.,Subcellular Location:
Cytoplasm .Expression:
Detected in brain cortex and lung (at protein level).
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