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GCSc-γ Polyclonal Antibody
Datasheet
Catalog Number:
E20-71883Amount:
100ulMolecular Weight:
72766DaObserved Band:73KDApplications:
WB;ELISAReactivity:
Human;Mouse;RatPurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human GCSc-gamma. AA range:266-315Source:
Polyclonal, Rabbit,IgG
- Western Blot analysis of various cells using GCSc-γ Polyclonal Antibody
Gene Name:
GCLCDilution:
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.Protein Name:
Glutamate--cysteine ligase catalytic subunitHuman Gene Id:
2729Human Swiss Prot No:
P48506Mouse Gene Id:
14629Mouse Swiss Prot No:
P97494Rat Gene Id:
25283Rat Swiss Prot No:
P19468Specificity:
GCSc-γ Polyclonal Antibody detects endogenous levels of GCSc-γ protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
GCLC; GLCL; GLCLC; Glutamate--cysteine ligase catalytic subunit; GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetaseBackground:
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010],Function:
catalytic activity:ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.,disease:Defects in GCLC are the cause of hemolytic anemia [MIM:230450].,enzyme regulation:Feedback inhibition by glutathione.,pathway:Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.,similarity:Belongs to the glutamate--cysteine ligase type 3 family.,subunit:Heterodimer of a catalytic heavy chain and a regulatory light chain.,Subcellular Location:
cytosol,integral component of membrane,glutamate-cysteine ligase complex,Expression:
Foreskin fibroblast,Hippocampus,Liver,Testis,Thalamus,
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