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Frizzled-7 Polyclonal Antibody
Datasheet
Catalog Number:
E20-71786Amount:
100ulMolecular Weight:
63620DaObserved Band:64KDApplications:
WB;IHC-p;IF(paraffin section);ELISAReactivity:
Human;MousePurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human FZD7. AA range:45-94Source:
Polyclonal, Rabbit,IgG
- Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Gene Name:
FZD7Dilution:
WB 1:500-2000;Flow Cyt 1:50-200;IF/ICC 1:100-500;ELISA 1:5000-20000Protein Name:
Frizzled-7Human Gene Id:
8324Human Swiss Prot No:
O75084Mouse Gene Id:
14369Mouse Swiss Prot No:
Q61090Specificity:
Frizzled-7 Polyclonal Antibody detects endogenous levels of Frizzled-7 protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
FZD7; Frizzled-7; Fz-7; hFz7; FzE3Background:
frizzled class receptor 7(FZD7) Homo sapiens Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. [provided by RefSeq, Jul 2008],Function:
domain:Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.,domain:The FZ domain is involved in binding with Wnt ligands.,function:Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogSubcellular Location:
Cell membrane ; Multi-pass membrane protein . Endosome membrane ; Multi-pass membrane protein . Associated to the plasma membrane in the presence of FZD7 and phosphatidylinositol 4,5-bisphosphate (PIP2). Localized in recycling endosomes in other conditions. .Expression:
High expression in adult skeletal muscle and fetal kidney, followed by fetal lung, adult heart, brain, and placenta. Specifically expressed in squamous cell esophageal carcinomas.
- Western Blot analysis of various cells using Frizzled-7 Polyclonal Antibody diluted at 1:2000
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