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FAST-1/2 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-71679

  • Amount:

    100ul

  • Molecular Weight:

    39257Da

  • Applications:

    WB;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human FOXH1. AA range:10-59

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using FAST-1/2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit

  • Gene Name:

    FOXH1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

  • Protein Name:

    Forkhead box protein H1

  • Human Gene Id:

    8928

  • Human Swiss Prot No:

    O75593

  • Mouse Gene Id:

    14106

  • Mouse Swiss Prot No:

    O88621

  • Specificity:

    FAST-1/2 Polyclonal Antibody detects endogenous levels of FAST-1/2 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    FOXH1; FAST1; FAST2; Forkhead box protein H1; Forkhead activin signal transducer 1; Fast-1; hFAST-1; Forkhead activin signal transducer 2; Fast-2

  • Observed Band(KD):

    40

  • Background:

    forkhead box H1(FOXH1) Homo sapiens FOXH1 encodes a human homolog of Xenopus forkhead activin signal transducer-1. FOXH1 protein binds SMAD2 and activates an activin response element via binding the DNA motif TGT(G/T)(T/G)ATT. [provided by RefSeq, Jul 2008],

  • Function:

    domain:The FM region is required for binding smad2/smad4 complexes. FM2 is more effective than FM1 and only interacts with phosphorylated smad2 that is in an activated smad complex.,function:Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).,similarity:Contains 1 fork-head DNA-binding domain.,subunit:Interacts with the MH2 domains of SMAD2 and SMAD3.,tissue specificity:Ubiquitous.,

  • Subcellular Location:

    Nucleus.

  • Expression:

    Ubiquitous.

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Catalog Number

  • Amount:100ul
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FAST-1/2 Polyclonal Antibody | EnoGene Biotech Co. Ltd