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Enolase Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-71561

  • Amount:

    100ul

  • Molecular Weight:

    47269Da

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human NSE. AA range:371-420

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Enolase Polyclonal Antibody diluted at 1:2000

  • Gene Name:

    ENO2

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

  • Protein Name:

    Gamma-enolase

  • Human Gene Id:

    2026

  • Human Swiss Prot No:

    P09104

  • Mouse Gene Id:

    13807

  • Mouse Swiss Prot No:

    P17183

  • Rat Gene Id:

    1.00912e+008

  • Rat Swiss Prot No:

    P07323

  • Specificity:

    Enolase Polyclonal Antibody detects endogenous levels of Enolase protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    ENO2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE

  • Observed Band(KD):

    47

  • Background:

    enolase 2(ENO2) Homo sapiens This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008],

  • Function:

    catalytic activity:2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O.,cofactor:Magnesium. Required for catalysis and for stabilizing the dimer.,developmental stage:During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.,function:Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.,induction:Levels of ENO2 increase dramatically in cardiovascular accidents, cerebral trauma, brain tumors and Creutzfeldt-Jacob disease.,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.,similarity:Belongs to the enolase family.,subcellular location:Can translocate to the plasma membrane

  • Subcellular Location:

    Cytoplasm . Cell membrane . Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. .

  • Expression:

    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

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Catalog Number

  • Amount:100ul
  • Price:225$

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Enolase Polyclonal Antibody | EnoGene Biotech Co. Ltd