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EKLF/CKLF/UKLF Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-71522

  • Amount:

    100ul

  • Molecular Weight:

    38221Da

  • Applications:

    WB;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human KLF. AA range:291-340

  • Source:

    Polyclonal, Rabbit,IgG

  • Western blot analysis of the lysates from K562 cells using KLF antibody.

  • Gene Name:

    KLF1/KLF5/KLF7

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

  • Protein Name:

    Krueppel-like factor 1/Krueppel-like factor 5/Krueppel-like factor 7

  • Human Gene Id:

    10661/688/8609

  • Human Swiss Prot No:

    Q13351/Q13887/O75840

  • Mouse Gene Id:

    12224/93691

  • Specificity:

    EKLF/CKLF/UKLF Polyclonal Antibody detects endogenous levels of EKLF/CKLF/UKLF protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    KLF1; EKLF; Krueppel-like factor 1; Erythroid krueppel-like transcription factor; EKLF; KLF5; BTEB2; CKLF; IKLF; Krueppel-like factor 5; Basic transcription element-binding protein 2; BTE-binding protein 2; Colon krueppel-like factor; GC-bo

  • Observed Band(KD):

    38

  • Background:

    This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. [provided by RefSeq, Oct 2009],

  • Function:

    function:Transcription regulator of erythrocyte development. Binds to the CACCC box in the beta-globin gene promoter and activates transcription. When sumoylation, acts as a Probably serves as a general switch factor for erythroid development. When sumoylated, acts as a transcriptional repressor, by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.,PTM:Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity.,PTM:Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity.,PTM:Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex.,simila

  • Subcellular Location:

    Nucleus . Colocalizes with SUMO1 in nuclear speckles. .

  • Expression:

    Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines.

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