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eIF3K Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-71510
  • Amount:

    100ul
  • Molecular Weight:

    25060Da
  • Applications:

    WB;ELISA
  • Reactivity:

    Human;Mouse
  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human EIF3K. AA range:61-110
  • Source:

    Polyclonal, Rabbit,IgG
  • Western Blot analysis of various cells using eIF3K Polyclonal Antibody diluted at 1:1000
  • Gene Name:

    EIF3K
  • Dilution:

    Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
  • Protein Name:

    Eukaryotic translation initiation factor 3 subunit K
  • Human Gene Id:

    27335
  • Human Swiss Prot No:

    Q9UBQ5
  • Mouse Gene Id:

    73830
  • Mouse Swiss Prot No:

    Q9DBZ5
  • Specificity:

    eIF3K Polyclonal Antibody detects endogenous levels of eIF3K protein.
  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Concentration:

    1 mg/ml
  • Storage Stability:

    -20°C/1 year
  • Other Name:

    EIF3K; EIF3S12; ARG134; HSPC029; MSTP001; PTD001; Eukaryotic translation initiation factor 3 subunit K; eIF3k; Eukaryotic translation initiation factor 3 subunit 12; Muscle-specific gene M9 protein; PLAC-24; eIF-3 p25; eIF-3 p28
  • Observed Band(KD):

    30
  • Background:

    The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).[supplied by OMIM, Mar 2008],
  • Function:

    function:Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.,mass spectrometry: PubMed:17322308,mass spectrometry: PubMed:18599441,PTM:The N-terminus is blocked.,similarity:Belongs to the eIF-3 subunit K family.,subunit:Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, wh
  • Subcellular Location:

    Nucleus . Cytoplasm .
  • Expression:

    Ubiquitous, with the highest levels of expression in brain, testis and kidney.

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Catalog Number

  • Amount:100ul
  • Price:225$

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eIF3K Polyclonal Antibody | EnoGene Biotech Co. Ltd