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E2F-1 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-71442

  • Amount:

    100ul

  • Molecular Weight:

    46920Da

  • Applications:

    IF/ICC;WB;IHC-p;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    Synthetic peptide from human protein at AA range: 100-170

  • Source:

    Polyclonal, Rabbit,IgG

  • Immunofluorescence analysis of rat-heart tissue. 1,E2F-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

  • Gene Name:

    E2F1 RBBP3

  • Dilution:

    IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300

  • Protein Name:

    E2F transcription factor 1

  • Human Gene Id:

    1869

  • Human Swiss Prot No:

    Q01094

  • Mouse Gene Id:

    13555

  • Mouse Swiss Prot No:

    Q61501

  • Rat Swiss Prot No:

    O09139

  • Specificity:

    The antibody detects endogenous E2F-1 protein

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    E2F1 RBBP3

  • Observed Band(KD):

    70

  • Background:

    The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can media

  • Function:

    function:Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.,PTM:Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.,similarity:Belongs to the E2F/DP family.,subunit:Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, ren

  • Subcellular Location:

    Nucleus .

  • Expression:

    Brain,Epithelium,Pancreas,Skin,

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Catalog Number

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E2F-1 Polyclonal Antibody | EnoGene Biotech Co. Ltd