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DNA pol β Polyclonal Antibody
Human;Mouse;Rat
WB;IHC-p;IF(paraffin section);ELISA
Polyclonal, Rabbit,IgG
Price on request
Quantity
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Gene NamePOLB
Product Information
Protein NameDNA polymerase beta
Human Gene Id5423
Human Swiss Prot NoP06746
Mouse Gene Id18970
Mouse Swiss Prot NoQ8K409
Rat Gene Id29240
Rat Swiss Prot NoP06766
SpecificityDNA pol β Polyclonal Antibody detects endogenous levels of DNA pol β protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NamePOLB; DNA polymerase beta
Molecular Weight38178Da
Observed Band(KD)38
Functioncatalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,cofactor:Binds 2 magnesium ions per subunit.,domain:Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity.,function:Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.,PTM:Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.,similarity:Belongs to the DNA polymerase type-X family.,subunit:Monomer.,
Subcellular LocationNucleus. Cytoplasm. Cytoplasmic in normal conditions. Translocates to the nucleus following DNA damage.
ExpressionSkin,Testis,
ImmunogenThe antiserum was produced against synthesized peptide derived from human DNA Polymerase beta. AA range:286-335
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
BackgroundThe protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011],
Western Blot analysis of various Immunohistochemistry cells using DNA pol β Polyclonal carcinoma tissue, Antibody. on the right is
analysis of paraffin-embedded human breast using DNA Polymerase beta Antibody. The picture blocked with the synthesized peptide.
Conjugated Antibody
This product is also available conjugated. Format: E20-713869-Conjugate. Price: $485 / Size: 100μl.
E20-713869-FITC$485 / 100μlE20-713869-Biotin$485 / 100μlE20-713869-HRP$485 / 100μlE20-713869-AP$485 / 100μlE20-713869-AF350$485 / 100μlE20-713869-AF405M$485 / 100μlE20-713869-AF488$485 / 100μlE20-713869-AF514$485 / 100μlE20-713869-AF532$485 / 100μlE20-713869-AF555$485 / 100μlE20-713869-AF568$485 / 100μlE20-713869-AF405S$485 / 100μlE20-713869-AF405L$485 / 100μlE20-713869-AF546$485 / 100μlE20-713869-AF594$485 / 100μlE20-713869-AF610$485 / 100μlE20-713869-AF635$485 / 100μlE20-713869-AF647$485 / 100μlE20-713869-AF680$485 / 100μlE20-713869-AF700$485 / 100μlE20-713869-AF750$485 / 100μlE20-713869-AF790$485 / 100μlE20-713869-APC$485 / 100μlE20-713869-PE$485 / 100μlE20-713869-Cy3$485 / 100μlE20-713869-Cy5$485 / 100μlE20-713869-Cy5.5$485 / 100μlE20-713869-Cy7$485 / 100μlE20-713869-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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