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DNA pol α Polyclonal Antibody
Human;Rat;Mouse;
WB;ELISA
Polyclonal, Rabbit,IgG
Price on request
Quantity
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Gene NamePOLA1
Product Information
Protein NameDNA polymerase alpha catalytic subunit
Human Gene Id5422
Human Swiss Prot NoP09884
Mouse Swiss Prot NoP33609
SpecificityDNA pol α Polyclonal Antibody detects endogenous levels of DNA pol α protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NamePOLA1; POLA; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180
Molecular Weight165913Da
Observed Band(KD)165
Functioncatalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,function:Polymerase alpha in a complex with DNA primase is a replicative polymerase.,miscellaneous:In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.,PTM:A 165 kDa form is probably produced by proteolytic cleavage at Lys-124.,similarity:Belongs to the DNA polymerase type-B family.,subunit:Interacts with SV40 Large T antigen; this interaction allows viral DNA replication.,
Subcellular LocationNucleus . Cytoplasm, cytosol . In the cytosol, colocalizes with RNA:DNA hybrids with a speckled pattern. .
ImmunogenThe antiserum was produced against synthesized peptide derived from human DNA Polymerase alpha. AA range:81-130
DilutionWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
BackgroundThis gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication. [provided by RefSeq, Mar 2010],
Western Blot analysis of various cells using Western DNA pol α Polyclonal Antibody diluted at cells, 1:1000. right is
blot analysis of lysates from HepG2, 293, and Jurkat using DNA Polymerase alpha Antibody. The lane on the blocked with the synthesized peptide.
Conjugated Antibody
This product is also available conjugated. Format: E20-713868-Conjugate. Price: $485 / Size: 100μl.
E20-713868-FITC$485 / 100μlE20-713868-Biotin$485 / 100μlE20-713868-HRP$485 / 100μlE20-713868-AP$485 / 100μlE20-713868-AF350$485 / 100μlE20-713868-AF405M$485 / 100μlE20-713868-AF488$485 / 100μlE20-713868-AF514$485 / 100μlE20-713868-AF532$485 / 100μlE20-713868-AF555$485 / 100μlE20-713868-AF568$485 / 100μlE20-713868-AF405S$485 / 100μlE20-713868-AF405L$485 / 100μlE20-713868-AF546$485 / 100μlE20-713868-AF594$485 / 100μlE20-713868-AF610$485 / 100μlE20-713868-AF635$485 / 100μlE20-713868-AF647$485 / 100μlE20-713868-AF680$485 / 100μlE20-713868-AF700$485 / 100μlE20-713868-AF750$485 / 100μlE20-713868-AF790$485 / 100μlE20-713868-APC$485 / 100μlE20-713868-PE$485 / 100μlE20-713868-Cy3$485 / 100μlE20-713868-Cy5$485 / 100μlE20-713868-Cy5.5$485 / 100μlE20-713868-Cy7$485 / 100μlE20-713868-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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