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DNA Ligase I Polyclonal Antibody
Human;Rat;Mouse;
WB;IHC-p;IF(paraffin section);ELISA
Polyclonal, Rabbit,IgG
Price on request
Quantity
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Gene NameLIG1
Product Information
Protein NameDNA ligase 1
Human Gene Id3978
Human Swiss Prot NoP18858
Mouse Swiss Prot NoP37913
SpecificityDNA Ligase I Polyclonal Antibody detects endogenous levels of DNA Ligase I protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameLIG1; DNA ligase 1; DNA ligase I; Polydeoxyribonucleotide synthase [ATP] 1
Molecular Weight101736Da
Observed Band(KD)133
Functioncatalytic activity:ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).,cofactor:Magnesium.,disease:Defects in LIG1 seem to cause immunodeficiencies and cellular hypersensitivity to DNA-damaging agents.,function:DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.,online information:DNA ligase entry,online information:LIG1 mutation db,similarity:Belongs to the ATP-dependent DNA ligase family.,
Subcellular LocationNucleus.
ExpressionBrain,Epithelium,Eye,PCR rescued clones,T lymphoblast,
ImmunogenThe antiserum was produced against synthesized peptide derived from human DNL1. AA range:111-160
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
BackgroundThis gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014],
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using DNL1 Antibody. The picture the right is blocked with the synthesized peptide.
Western Blot analysis of various cells using on DNA Ligase I Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute Cytoplasmic and Nuclear Fractionation kit.
Conjugated Antibody
This product is also available conjugated. Format: E20-713864-Conjugate. Price: $485 / Size: 100μl.
E20-713864-FITC$485 / 100μlE20-713864-Biotin$485 / 100μlE20-713864-HRP$485 / 100μlE20-713864-AP$485 / 100μlE20-713864-AF350$485 / 100μlE20-713864-AF405M$485 / 100μlE20-713864-AF488$485 / 100μlE20-713864-AF514$485 / 100μlE20-713864-AF532$485 / 100μlE20-713864-AF555$485 / 100μlE20-713864-AF568$485 / 100μlE20-713864-AF405S$485 / 100μlE20-713864-AF405L$485 / 100μlE20-713864-AF546$485 / 100μlE20-713864-AF594$485 / 100μlE20-713864-AF610$485 / 100μlE20-713864-AF635$485 / 100μlE20-713864-AF647$485 / 100μlE20-713864-AF680$485 / 100μlE20-713864-AF700$485 / 100μlE20-713864-AF750$485 / 100μlE20-713864-AF790$485 / 100μlE20-713864-APC$485 / 100μlE20-713864-PE$485 / 100μlE20-713864-Cy3$485 / 100μlE20-713864-Cy5$485 / 100μlE20-713864-Cy5.5$485 / 100μlE20-713864-Cy7$485 / 100μlE20-713864-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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