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DGK-ι Polyclonal Antibody
Human;Rat
WB;IF/ICC;ELISA
Polyclonal, Rabbit,IgG
Price on request
Quantity
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Gene NameDGKI
Product Information
Protein NameDiacylglycerol kinase iota
Human Gene Id9162
Human Swiss Prot NoO75912
SpecificityDGK-ι Polyclonal Antibody detects endogenous levels of DGK-ι protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameDGKI; Diacylglycerol kinase iota; DAG kinase iota; Diglyceride kinase iota; DGK-iota
Molecular Weight(Da):116997Da
Observed Band(KD)160
Functioncatalytic activity:ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.,similarity:Belongs to the eukaryotic diacylglycerol kinase family.,similarity:Contains 1 DAGKc domain.,similarity:Contains 2 ANK repeats.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,
Subcellular LocationCell projection, axon . Cell projection, dendrite . Cell junction, synapse, presynapse . Cell junction, synapse, postsynapse . Cell junction, synapse, postsynaptic density . Cell junction, synapse, synaptic cell membrane . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane . Cytoplasm, cytosol . Nucleus . Excluded from inhibitory synapses (By similarity). Localization between cytoplasm and nucleus is regulated by protein kinase C (PubMed:9830018). Both in the detergent soluble and particulate fractions (By similarity). .
ExpressionSpecifically expressed in brain and retina (PubMed:9830018). In brain, highly expressed in hippocampus, caudate nucleus, occipital pole, cerebral cortex, and cerebellum (PubMed:9830018). Also detected in kidney (PubMed:15894621).
ImmunogenThe antiserum was produced against synthesized peptide derived from human DGKI. AA range:991-1040
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
BackgroundThis gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. [provided by RefSeq, Jul 2008],
Western blot analysis of lysates from 293 cells, using DGKI Antibody. The lane on the tissue. right is blocked with the synthesized Sodium peptide. 3,Secondary
Immunohistochemical analysis of paraffin-embedded human spleen 1,primary Antibody was diluted at 1:200(4 ℃ overnight). 2, citrate pH 6.0 was used for antigen retrieval(>98°C,20min). antibody was diluted at 1:200.
Conjugated Antibody
This product is also available conjugated. Format: E20-713838-Conjugate. Price: $485 / Size: 100μl.
E20-713838-FITC$485 / 100μlE20-713838-Biotin$485 / 100μlE20-713838-HRP$485 / 100μlE20-713838-AP$485 / 100μlE20-713838-AF350$485 / 100μlE20-713838-AF405M$485 / 100μlE20-713838-AF488$485 / 100μlE20-713838-AF514$485 / 100μlE20-713838-AF532$485 / 100μlE20-713838-AF555$485 / 100μlE20-713838-AF568$485 / 100μlE20-713838-AF405S$485 / 100μlE20-713838-AF405L$485 / 100μlE20-713838-AF546$485 / 100μlE20-713838-AF594$485 / 100μlE20-713838-AF610$485 / 100μlE20-713838-AF635$485 / 100μlE20-713838-AF647$485 / 100μlE20-713838-AF680$485 / 100μlE20-713838-AF700$485 / 100μlE20-713838-AF750$485 / 100μlE20-713838-AF790$485 / 100μlE20-713838-APC$485 / 100μlE20-713838-PE$485 / 100μlE20-713838-Cy3$485 / 100μlE20-713838-Cy5$485 / 100μlE20-713838-Cy5.5$485 / 100μlE20-713838-Cy7$485 / 100μlE20-713838-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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